I program and run a laser for a living. Sometimes I get a drawing that I need to create my programs from, sometimes a dxf. The cad file is the best, takes me the least amount of time.
Some places charge for initial artwork/setup, and some don't. Time in cut and material cost are the biggest factors. Quantity does have some affect, but not as much as with punching.
If you're wanting that cut from o-1 or a-2 that is .750 thick, that will be expensive just for material. Most of the stuff I run is mild steel, stainless steel, or aluminum. Let's say that it's a relatively small piece of steel, only 2' by 2'. (I usually run 5' x 10') Now that piece of steel weighs about 122 pounds at .750 thick. Say you got a good deal for o-1 at $2 per pound, there's $244 for materials alone! The $300 doesn't sound like that bad of a deal.
Jamie